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Flow Cytometry Examples |
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| Paroxysmal Nocturnal Hemoglobinuria (PNH) The
classical demonstration of PNH by complement-mediated hemolysis (Ham’s Test and
Sucrose Lysis Test) is now known to reflect a deficiency in GPI-anchored proteins,
including CD59 (membrane inhibitor of reactive lysis) and CD55 (decay accelerating
factor). |
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The use of monoclonal antibodies against CD55 and CD59 in the
analysis by flow cytometry of red cells and granulocytes provides the clinician with a
powerful new tool to detect even small populations of blood cells partially or totally
deficient in these proteins. Flow analysis of erythrocytes and granulocytes provides the most accurate diagnostic test for paroxysmal nocturnal hemoglobinuria (PNH). This technique may also be valuable in the evaluation of patients with aplastic anemia. |
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| Specimen Required: One 5 ml EDTA tube of
whole blood. Forward promptly at ambient temperature. DO NOT FREEZE OR COOL SPECIMEN.
Specimens received within 48 hours are suitable. |
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Transport: Please call (612)220-8910 for specimen transport assistance. Turnaround Time: One day, Monday through Friday. Figures 1 and 2: CD59 expression measured by flow analysis of granulocytes and erythrocytes. Patient with known PNH. |
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| This page was last modified December 03, 2004 Copyright © 1997-2000 Hospital Pathology Associates, P.A. |
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